LOCUS BG069603 738 bp mRNA linear EST 12-MAY-2010
DEFINITION H3078B05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3078B05 3', mRNA sequence.
ACCESSION BG069603
VERSION BG069603.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 738)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069603.1.
Other_ESTs: H3078B05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3078 row: B column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..738
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3078B05-3"
/db_xref="taxon:10090"
/clone="H3078B05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 242 a 136 c 149 g 211 t
ORIGIN
1 gttaatttca tcatacatat ttacttaatc tctacagaat tgagtagata aaatcagatt
61 tacggtagtg aaaaatctct acaaaatgtc tttgaaaagc aaaacaagac tgcctcttaa
121 caattcatat cctcatgaaa gacgtgggct gtaaaaataa agcagtgtgt ttagcacaaa
181 ctatatctgc atcatttttt cacagttatt tccatctaca gtctgaaaga ggtagatttt
241 tttttctgca acacctgaga gttgaattgt gataaccagg tgtgtaataa aggcagttgc
301 atacataact aaaaaataca ggtctcaaga ccagaaaatg tacttctaca agtctgagga
361 tgaaatggcc tgatacccaa gtttcgctac attttggggt ctactgacac taaaaatata
421 catcaagatt gcatggtagt gcaccatatg cagtaagtga attcaacata gaaatctttt
481 ctgggccagc acagtagcat tacagtgttc tagaatacgg tagtacaggg cgtgctacaa
541 agccacaaca ctgggctgct gaggtgatca ctttaaggga gggagggagg gcatccttct
601 ttgaggtccc atctgttttc attcacagga ggagagattt tgcatatgca tatttcatac
661 agaatctctg tcctctaaag atctagctga aaatgcatgt aagccgaaag cagcaggcca
721 ggactgttaa tcgcattg
//