LOCUS BG069469 783 bp mRNA linear EST 12-MAY-2010
DEFINITION H3075G05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3075G05 3', mRNA sequence.
ACCESSION BG069469
VERSION BG069469.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 783)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069469.1.
Other_ESTs: H3075G05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3075 row: G column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..783
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3075G05-3"
/db_xref="taxon:10090"
/clone="H3075G05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 231 a 163 c 188 g 201 t
ORIGIN
1 ccttcctctg agatgggggt cccatcatac tgtgtagact tggatgcctt ggggcatgct
61 gtatagacca gactggcctc aaactcatag agatccacct gcctctgtcc cctgagtgat
121 gggggaaagg cccttgccac caagcctggc ttacgtggct attttgagca ttaaatttgt
181 aaacaaataa atgtgttgct tcatctcagg aaactgcttc tgtttctgca ctcctcttta
241 tcactgcaaa gtaagggaaa aggcgtaatg gacagaataa gcgggaggta catgcagaat
301 tgtgacaccg tgtgacagtg cagttgtaag gaccacttta tgggtttata tatagatatt
361 ggtagactgg gtacacaggt cattcggtag ggagcttgtt tagcttatgc aaagccctga
421 atcccatcct cagtattgcc taaatcaggt atagtggagc acatctgtaa tcccaacaaa
481 tgagaagtga aaataggaga ttcacaagtt caagggcatc cttggagttc aagactagcc
541 tggaatacat aagaccctgt ctcaaaaaag agagggtgac ttgggggaga atagagaggt
601 ggttaagagc actggctgct cttccagagg tctcaagttt atttccagca ccagtctgtg
661 actccagttc caggtgatcc aaaaccctct tctggcctct aagggcagtg gacacacatg
721 gtatacagac ataatctcaa gcagacataa aacaaaaata agtgaaatct tttaaaaata
781 aag
//