LOCUS BG069465 565 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075G01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075G01 3', mRNA sequence. ACCESSION BG069465 VERSION BG069465.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 565) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069465.1. Other_ESTs: H3075G01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3075 row: G column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..565 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075G01-3" /db_xref="taxon:10090" /clone="H3075G01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 158 a 147 c 112 g 148 t ORIGIN 1 aggtctgaaa aaataatccg tttaattgaa aaacctggag aatccactcc atgaaaagac 61 cagacgcccc ccacccctac attacttcat agccacttcg aatactcttc accagacagc 121 aggagaagat aattcccaag acctccacaa aagcaatgcc cagggccgct gcagccacca 181 gcagtatgtt cttccttagc catattgcta tagtctccac gcagccctgg gtatggatag 241 tggattcctt gaaatcattc ccacagccca cagttatgtt gatgcagcaa gaatcgggga 301 ctctgtcctt ggccatgccg gggatgtttt cccagtctgt gtagttagaa gctccacagc 361 agttattttc tttctgcaat ttgtccaaaa tagtggctgt tttgttgtct ttaaggtaat 421 tctgcatctg ctgctggaag cttttattaa actctgactt cacctggtct ctaaacacat 481 agccagcaat ggccacagcc acctccacaa gcatgataag agacaggaag atggcaaatg 541 taatcatgag acagtagttc tcctt //