LOCUS BG069436 763 bp mRNA linear EST 12-MAY-2010
DEFINITION H3075E07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3075E07 3', mRNA sequence.
ACCESSION BG069436
VERSION BG069436.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 763)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069436.1.
Other_ESTs: H3075E07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3075 row: E column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..763
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3075E07-3"
/db_xref="taxon:10090"
/clone="H3075E07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 206 a 191 c 191 g 175 t
ORIGIN
1 gagtacactg tagctgtctt cagacacacc agaagagggc atcagatctc attacggatg
61 gttgtgagcc accacatggt tgctgggatt tgaactcagg accttcggaa gagcagtcag
121 tgcctttaac cgctgagcca tctctccagc ccaagaccca cttcctatag tcagaagaca
181 tgctgtggga gtctgctggg catgccctct gcacctcctc tggtgacaac caactcatgc
241 ctcgctctcc ctcccatcct gctctgccag gaagattggt tccagagggg atgctctggg
301 tcagacccac tcagtgaagt atttagagat gacaggagaa gtccaaggct caattccgta
361 gagggaccat cccttgtttg cagagcaagg aggggcgaac gaagatgtat tgctatttta
421 agaggagcag tcttggggtc caagagatgg ctcagaggtt aagagcgctt gatattcttg
481 caggggacta ggtggtatat acacactgca gctcactagg gttccatttt ttgtacacac
541 acagcagctc actatcatct ttagctctag tcccagggaa tctaacaccc tccacaggta
601 tgaggcatgt acagagcaca tctaagcatg caggcaagac tcctaaacac agtataaaca
661 aaacaaaatt gtaaacgaaa aagagcctgg ggacatattg ggccatgtat aaactggagg
721 gctttaaatc ctccctccac aagctgtagg tggagcagct gcc
//