LOCUS BG069426 777 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075D09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075D09 3', mRNA sequence. ACCESSION BG069426 VERSION BG069426.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 777) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069426.1. Other_ESTs: H3075D09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3075 row: D column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..777 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075D09-3" /db_xref="taxon:10090" /clone="H3075D09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 222 a 188 c 199 g 168 t ORIGIN 1 ggtataaaag taggaacaac gagctgcatg aaactctgct gtggcaactg agtgcatttg 61 ccctgcggct ctaatgtgag ggtcagaggc tagtcagtga ccggcagcag caagggtttg 121 cctgaggagc agctggcacg tttcaccttt gtgtcaggta gttacacacg ggaacaggag 181 ttaggagagt cagatggcca agcatcagat acggtttcaa gcaaaccgtg tccttaaagc 241 tgagccgaca agagaaccct ggtgatgaag gtggaactca ccgagctaga gtcagacagt 301 catcgtgagt tcccagtttg gtcaaggtcc aacaacagga ctaaaacgag acgggcagac 361 tctgatagag actggagaga ggtttgcttc taagaaagat acccggctta gatgcacccg 421 ctcacagatc tcacagtttc aaatgcagaa aaccgaaagg tagttaccat ctttctgaca 481 ttgccaaggt gggagagcag acgctctgcc aactcagccc agtaactcag cccctgcctc 541 cccactatga aaagggagca gaggcaaata ggtccggccc ctgcggggga ccagaacctc 601 aacagcttag gctactatgc aacttcccag agtcgttctc caaaagcctg tgtacttatc 661 taggaattgg taggtcaaat acttgatact ttggcaaata atttattcaa tactctttaa 721 agtccctctc aaaaagtctc agcacggtgc tgcgtcacac tgctggccac aagttaa //