LOCUS BG069398 744 bp mRNA linear EST 12-MAY-2010
DEFINITION H3076H07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3076H07 3', mRNA sequence.
ACCESSION BG069398
VERSION BG069398.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 744)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069398.1.
Other_ESTs: H3076H07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3076 row: H column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..744
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3076H07-3"
/db_xref="taxon:10090"
/clone="H3076H07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 189 a 171 c 140 g 244 t
ORIGIN
1 gagacaggat ttctctgtgt agccctggct gtcctggaac tcactctata gaccaggctg
61 gcctcgaact cagaaatccg cctgcctctg cctctcgagt gctgggatta aaggcgtgcg
121 ccaccacgcc cagcatattt acatttttta tctataaaga tatacacaga acagaatctt
181 ttgcttatta gtttgttttt aaacaacccc caaaactccc caccaccacc gtgtaagctg
241 tcttgcaatc tgtgatccct ttactcggcc cccattgcta ggatcacagg agtgttagtg
301 tgcagaaagc tagcagagct cagtggtttt cactttgggt tttttttttt tcttgttttt
361 gttttgtttt attttgttgt ttttaaaatg gggtctttac tatatagctc tggctgaata
421 catgtcacta tacagaccaa gctagtcttg tctggaattc aaactcacaa agatcctcct
481 gcctcggctt ccagagcact agagttaaag gcatgcacta ccatgcctgg ccccaaatcc
541 ttttatatta cttacagggg ggaaagtaga actttctctt aaaagctaca agttttgatg
601 tcaggatatt tattaactat aataatgata agatcactga tcatcaatct atgccaaccc
661 ttttctgtat acttggtttt tatggtactg agaatcaaat tctgggcttc aagcttgcta
721 agcaagagtt ctaccattgg accc
//