LOCUS BG069391 753 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076G12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076G12 3', mRNA sequence. ACCESSION BG069391 VERSION BG069391.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 753) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069391.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: G column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..753 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076G12-3" /db_xref="taxon:10090" /clone="H3076G12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 202 a 172 c 168 g 211 t ORIGIN 1 cacggtttaa atagaattta ttcatttttg acaatttcat gcatgtaaac aatccatctt 61 gatcattggc accccgacac ccccttcaac tcctacaact tccttcaata tgacgctctc 121 cctcaaaaag tagattttgg ggagggggtg ggttagggag tgggacagct taagcaactg 181 tgtgcttagt gtcccaatgt ttaccttaga agtaacgggt cctagggctg ggggacaaga 241 cataaaacca cttaacagac agttccaatg ggtttcatat ccctgtcatg aagaaaggta 301 agcccaatgt acaaaataat actggagcaa ttgttagcca cagagagaaa ccccaaagca 361 ggtggaatct agcaggatgt gtattgaggg cttttggtgc tgcttctaga atttggcatt 421 tctccctggg ctccaacaag aaagtatgct cagataagaa tattagaaac agtgaccatg 481 ggactttacc accaccttcg gccatctcct tgcctactac gttacttact accctaagtg 541 atcttcttgt tcactacttg gcttgcttac tttgtctttg ctctaagagc tgaccacgtt 601 ttttggatgc acctaggagg atataaagca ggctggagag aaatgaagct gcttcagtct 661 caggacaggg tggagtcagg tgatcatgct gtctaacgtt ttctcctcaa tcctcactcc 721 ctggagaccc tgctgactga ctgaactggc ttg //