LOCUS BG069290 646 bp mRNA linear EST 12-MAY-2010 DEFINITION H3074G07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3074G07 3', mRNA sequence. ACCESSION BG069290 VERSION BG069290.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 646) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069290.1. Other_ESTs: H3074G07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3074 row: G column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..646 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3074G07-3" /db_xref="taxon:10090" /clone="H3074G07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 197 a 108 c 115 g 226 t ORIGIN 1 aaatattctc tcataagttt ccagaaaaac atcacatgat acaattgatt cttcaaagaa 61 actagaaatt cccacttcag tttgaccacc ttgtaagtac attgtagatg tatttttatt 121 tttgacaaag ggtctcacta cgtagctcag gctatcttgg ggcttactat gtagaggctg 181 gccttaaact cagagagatc tgatctgtct gattttgctt cctgagtgct ggaagtaaag 241 gtgtttacca ctgcatctag ccatattgta gatttcttca gtttgagaaa agtctaccat 301 ttttgttcat attttatatt tgtttgtatt ttgtcagatt tggcatgtat tagaaggtat 361 tcacttaaaa gaaaaaattg tcttttttcc cttaaatcag ctccctatga acttctgtga 421 actgtttggg tgtcatgagc cactggacct gtaagcactc agtgtgagtt ggtgttagaa 481 ttatagaata ctgtagctcg atataagcaa ttataaagca agtataaaca aatataaagc 541 aaaatatgaa gcactatttt aactttagtc tctacctgtc ctagctacta tgacaacatg 601 gcaatactaa gcataatatt tttaaatagt gactttggag ggctta //