LOCUS BG069268 625 bp mRNA linear EST 12-MAY-2010 DEFINITION H3074E09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3074E09 3', mRNA sequence. ACCESSION BG069268 VERSION BG069268.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 625) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069268.1. Other_ESTs: H3074E09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3074 row: E column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..625 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3074E09-3" /db_xref="taxon:10090" /clone="H3074E09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 259 a 113 c 100 g 153 t ORIGIN 1 ggcaaccatc tttttattat ctaaagacaa caccaatatc tcattcagaa aaaaataatt 61 ctagctagat tgtgccccct atgtgaaaga caaaatatta gaacttaaag ttatataaaa 121 taatctcttt ataatttgaa gaaagcaaag attatttaat ctacaaagat tgacgcacta 181 tataaaattg agaacaccta ggagtaaatt tcattataaa agagtaaaag caaactatgg 241 actgggaaat atttttaata tacacatcta tataaagttc ccatcccata ccataaaaag 301 aatgaccaca aatccagact tactctctaa agcaaactgc taatagaaaa cggggcaaga 361 gactggatca ggtaacactg tatgcataga atgaagccaa ctaggtaata agcacaggga 421 gagatgttta ctctcactaa tgatcagaaa aaaatgtagc taaatatcaa aaggaaacat 481 tgctaagcat tcatcaagtg gctaaaacta aaggcttgac aatgtcacac tctgggaaaa 541 ccagatgtaa catatgtaac atagctatga ccagcacaaa ttagaaggcg ctgcctagga 601 cactgcagag atcacgccct cagaa //