LOCUS BG069231 639 bp mRNA linear EST 12-MAY-2010 DEFINITION H3074B08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3074B08 3', mRNA sequence. ACCESSION BG069231 VERSION BG069231.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 639) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069231.1. Other_ESTs: H3074B08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3074 row: B column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..639 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3074B08-3" /db_xref="taxon:10090" /clone="H3074B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 150 c 127 g 201 t ORIGIN 1 gctttatcca aagttttact tctgtaacaa acaaggccag gaaatggaga agctaaaaac 61 atacatgaaa tttcaagacc cccctcgata ccctgctgcc aatcagtccc gagtcttcaa 121 aagcctcagg tgatgacgta tcgggtaagt cctccccctc ggacctctga cagtctgtgg 181 caatgccgca aaatgtgcat aatcacataa aacctcttgt caactttcaa agccgtgaat 241 tcctttatgt cagcttccac gatgaagtga tgacctttcg tgtccttcgt ttcttcatta 301 ataaatctct ggtagagatt tctcttcaca gagcccatag atgcttttgg ttgatgcata 361 atcttatatt tgctaactac tgccttgtta atttccttaa taatatcatt aatttgacaa 421 tatgttaaac gggatttcat gtacgcagaa cgtcactgaa ttcatccata gttataaatt 481 gcatttcctt gataatcctc tgctctctgg ggggcttctt cggcagtcca ggctcctcag 541 ctttgtctga ctctttagga tccggctctg gcttgtgaac taagctctgg gtcgccgtca 601 cttgaggcag atggggaggg acgtgttctt tgagatgtt //