LOCUS BG069095 693 bp mRNA linear EST 12-MAY-2010 DEFINITION H3072F09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3072F09 3', mRNA sequence. ACCESSION BG069095 VERSION BG069095.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 693) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069095.1. Other_ESTs: H3072F09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3072 row: F column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..693 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3072F09-3" /db_xref="taxon:10090" /clone="H3072F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 167 a 195 c 196 g 135 t ORIGIN 1 cagagttctg gccaaaaaga aaagaagctg aatagtaaag attcttcttc tccaaccaag 61 cagagaaagt cataaatctc cgccctcggc cgcagaaggg caaaggcgag tctgctgcca 121 ccagatgatt tgaactgtcg aaagacctca gcacacagaa aggccagcca ttagtggtga 181 agggggaaac gcaggaaagg aatgctttaa tcacaggttg cggccacagc ccctgcaagc 241 ttctgacacc tgcctgagca ggagcgcatc cctctgtggg cttccggagg ccccttggag 301 gacacccgcc caccccagtc agagggctcc acatcctttc ggaattctct tagtcacaga 361 ctcttgaagc ttgagctcag gcgaggtggg gtgcccggac tgaggcttag acggtgatct 421 gttgattttc tctaaaaaag ggtctctggt gtgaggtaca gatatccgga aacatgcgga 481 aagcctggtc cggctggggc tcctccaggg ctggcatcag ggagccagca ccgcagctct 541 tgcccgcttc ctccaccagc tgcctggcat acagctgctc tacctgcacc agaatgagtt 601 tggagcgcaa gggcacagtg tccggaaact cctccccgaa gcacaggacc accctgctgt 661 caggtaggcg gctctgggtg gcgtagaatt gct //