LOCUS BG069062 669 bp mRNA linear EST 18-DEC-2003
DEFINITION H3072C11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3072C11 3', mRNA sequence.
ACCESSION BG069062
VERSION BG069062.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 669)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT On Jan 26, 2001 this sequence version replaced BG069062.1.
Other_ESTs: H3072C11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3072 row: C column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..669
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3072C11-3"
/db_xref="taxon:10090"
/clone="H3072C11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 180 a 148 c 138 g 203 t
ORIGIN
1 gggactttta tcttctacat aaatgtgtag atacacggtc acttatgtgc atgctgtcag
61 tccattttct tgtacagaaa aaagtgtttg tagaagcaca cacgtggtta tacacttaca
121 ctttttttct gtaaatattt gttcttaagc gttgcacatg actcgtttgt tgacacacag
181 ttttctgcgc ccctcacgtc tttctttcat gttgctgcag ttgtgctgct tgattccttt
241 gactctgttg aagatgttgg ccttagcagt caagagaaag caacccctaa agcaaaggcc
301 cctccagaaa taacagacga cggaccagca gaaacaggta aagtatgtga ggccctgact
361 cttctctaat cactacagga ttctctgtga gcaactggct gcatttctag aaaaatagat
421 gaaataacca tctagacatt agagacactc attctcatag caatgtctgg caatacaggg
481 gagtgctgtg gctaagagtt agctacccac gatgctcacc atgtgtcggg gaaatgggac
541 atgggtctcc ctatgcattc tgtagttctc tccttagcag caggctaggt cacgccctta
601 aattctgaac tagtttcctt acagctagta acagttttgt gataatgcag tagatctatc
661 attaccaat
//