LOCUS BG068800 729 bp mRNA linear EST 12-MAY-2010
DEFINITION H3069D11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3069D11 3', mRNA sequence.
ACCESSION BG068800
VERSION BG068800.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 729)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068800.1.
Other_ESTs: H3069D11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3069 row: D column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..729
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3069D11-3"
/db_xref="taxon:10090"
/clone="H3069D11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 190 a 184 c 122 g 233 t
ORIGIN
1 ccttccaagg ctgcttcttc cttcatgcct tagccatctt acaaggagct attgtggcaa
61 taattaattt taaccagggg gcttctaccc caccttcaat catttagttc ccacattaaa
121 aacacaaaac tttgataatt ataagaagcc ttaatcagca ctagatttgg gtaggtatta
181 actctcttat gactattttg tctgcttccc tgtaaataat cccaagatat gacttgccat
241 gttccatctg ggctactctt aactccaatt ggctatccct catggctagt tctcatgatc
301 ccttaccaca tggtgtcttc tcctttctcc accttctttc tctccccttg tggtcctgcc
361 tctggtctca agcccaggga actgaagcct catctatgtc tctcctgccc agatataagc
421 tgtaggcatc tttattcaac caatagtttt aaattaatga gcaaggtttc atggcatcat
481 ttggggtaca tgaggattcc ttcatcccta aggtaaccat atctctggag ccatttagca
541 tttcaataca cagcaaagac caaaccacga gtagctgtta actgtttcca gaacataacc
601 tcccagattc ataggcattg gagggaatgg ctatgggtcc caggaactca agacaagagt
661 tttagaatta agtctcatta tctctcattg gtctaatttg agtcatttct gtcagccaat
721 cactgtcag
//