LOCUS BG068674 777 bp mRNA linear EST 12-MAY-2010 DEFINITION H3068B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3068B04 3', mRNA sequence. ACCESSION BG068674 VERSION BG068674.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 777) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068674.1. Other_ESTs: H3068B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3068 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..777 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3068B04-3" /db_xref="taxon:10090" /clone="H3068B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 170 a 226 c 201 g 180 t ORIGIN 1 cattaggaaa cctttattgt caccattaga gataaaaata tcggagtccc acacaccaca 61 ccccacgagg aggataactg aagaagggtt actgattgtc acagaagccc aggcataggg 121 ctgaacatcc gtcctcggtc acccccagct tccagccacc ccctcccttg cacgggcagc 181 aggcagcagg tcagtggcag agggcgatgg tctccatgtt gaggaaacga atgtgcatct 241 tggtctcgat gtcgatgccc tgccagatct tcaggaagtc ctcaaaggtg atcccctcgt 301 acacctggtc cggttccatc tgccccacgc acacgctggc cgcctccatc atggccccgt 361 ctgcaatgga ccgagccgac tccttttcaa tgtgagggtt tcccgagagc agctcctcca 421 ccacatttct atactcttcc agggtgatgc ggccgtcact gtccgaatca tacatatgaa 481 acagaaattt cagcttctcc tttcgtgaca gctccacctg ttcctcgccc agggtggtgt 541 cgatgggccg gaagtaggac atgatagtca ggaagtcctc aaagttgatc tcatcggcca 601 gaccgctgga tccctttcgc aggttcctgt tgtcgaagaa ggcacggacg attttggatc 661 ggatcgggtt gagctccagg tcagggacat tgttgaagtt ctccttgcga atggtgggct 721 ggtcccgctt agctgcttga acctccgatg cagctgctct atctggtccg aggagaa //