LOCUS BG068549 523 bp mRNA linear EST 12-MAY-2010
DEFINITION H3066G06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3066G06 3', mRNA sequence.
ACCESSION BG068549
VERSION BG068549.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 523)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068549.1.
Other_ESTs: H3066G06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3066 row: G column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..523
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3066G06-3"
/db_xref="taxon:10090"
/clone="H3066G06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 136 a 138 c 136 g 113 t
ORIGIN
1 cttttattcc aaaagccttt aattgcattt ctcaatgttt ttcaaaaaca aaaagatcca
61 aattataggt aaaaaggaac atggaaaatt caactcaagt ggtgtcacga tcttggggtc
121 acgcttcagg catggtagga cccaagaagg cacactacgt gccctgggcc cgcctgaccc
181 tgctggttac cacaatcagt agagcactgc ccgtgagccc tgctgcatac ctccctccac
241 acaccagaga ggaaggcctg agcagggaaa cagcagacat ggccctgcct tgacctaaac
301 ctgccaactg gccttcagca tcacctcatc cctctttaaa tggctaaggg aggctagggg
361 tcacaaggcc gtgtaacatt aggctgaggc ggcgggcagc tttgctaagt gagttgtgtg
421 gatcagcctg caggaactgg aagagcttct ggcgcacctg agccatgaca gagcccatgt
481 ggtctcgagt gatgggatcg ctgtggtcca ggtgcatcac agc
//