LOCUS BG068536 536 bp mRNA linear EST 26-JAN-2001 DEFINITION H3066F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066F04 3', mRNA sequence. ACCESSION BG068536 VERSION BG068536.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 536) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3066F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..536 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066F04-3" /db_xref="taxon:10090" /clone="H3066F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 119 c 116 g 135 t ORIGIN 1 gctgtaggac ccttcgtttt cagccctagt acttgttagg gaaacagcct catactcaga 61 aacctgcctg cctctgcctc taccaatttt tccagagtgc tgccattaaa ggaacgtgcc 121 tccattaaat gttgggtttt ctacatcttt ccttggtcat ggtgtctgtt ctctgctgta 181 aagccttaac taagacatgt agtgagatct aggtcaccca gagctacaca gtgagaccaa 241 tggaatagaa ttgaagaccc agaaatgaac ccacacacct atggtcactt gatcttcgac 301 aagggagcca aaaccatcca gtggaagaaa gacagcattt tcaacaattg gtgctggcac 361 aactggttgt tatcatgtag aagaatgcga atcgatccat acttatctcc ttgtactaag 421 gtcaaatcta agtggatcaa ggaacttcac ataaaaccag agacactgaa acttatagag 481 gagaaagtgg ggaaaagcct tgaagatatg ggcacagggg aaaaattcct gaacag //