LOCUS BG068464 577 bp mRNA linear EST 12-MAY-2010 DEFINITION H3065G11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3065G11 3', mRNA sequence. ACCESSION BG068464 VERSION BG068464.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 577) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068464.1. Other_ESTs: H3065G11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3065 row: G column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..577 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3065G11-3" /db_xref="taxon:10090" /clone="H3065G11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 123 a 143 c 157 g 154 t ORIGIN 1 gttttttgtt ttaaactaca gagaacctca gcaaatgccc aaatgccatg gagttacagt 61 caagtgtgac aggaaacttg acacttgcat gtggaatcct gagaaatcga atgagacatt 121 ggcagaccgg gtttcttaag gggttgggtc atatgcagcg ttagtgaaga ctccgtatgt 181 tcctaggtgg ctgtgagggt tcaaggggca ttttccatca tctttctttc tgagacgctt 241 tcttagcaga gccaggagtg tcccgcccac tgccaggcct gttgtagaag ccactgcacc 301 acccaagtac gtcagagctt cctggattgt cagtgtccct gcttggcagg ttggcatggc 361 atctgaccag tgaccgtcct ctcggcaggt tgctctcaca gagccgttca gtgactgccc 421 ctctgggcac tggaaggtgc aggttgatcc atagctaaag tttccccagg gattggaaca 481 gttcattgct actgctgtgt ccatgtgcaa ttcggagcat ttgaccgctc tgcacatggg 541 ggtcactgct gtccattgtc ctgaagccct gcagcgg //