LOCUS BG068448 744 bp mRNA linear EST 26-JAN-2001 DEFINITION H3065F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3065F04 3', mRNA sequence. ACCESSION BG068448 VERSION BG068448.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 744) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3065F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3065 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..744 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3065F04-3" /db_xref="taxon:10090" /clone="H3065F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 289 a 155 c 136 g 164 t ORIGIN 1 ggacaaagat gccaaaacca tacggtggaa aaaagaaagc atccttaaca aatggtgctg 61 gtgtaactag atatctgcat atagaacact gcaaatacat ccatatctat taccctgcac 121 aaaactcaag tccaagtgga tcaaagacct cacacaaaac ctgatacatt aagtttaatg 181 gaagataaag tggggaatcg ctttgaatgc attggtacag ggcctaactt gtttaacaga 241 acaccaatag ctcaggccct aataccaaca attaataaat gaaacctcag aaactgaaaa 301 gtttctgtaa ggcagagggc actgtcaatc ggaaaaagca gcaatctaca gactgggaaa 361 gatctttacc aaccttacat ttgacagaag gttaatatgc aaaatatata aagaactcaa 421 gaaattaaac actaccaaac caaataaccc aattcaataa tggatcacag agctgaacag 481 aattctcaac aaagaaatct ctaatggtca agaagcactt aaagaaatgt tcaacatctt 541 tagtcatcat agaaatacaa atcagaacaa ctccgagatt ccaccttaca ccaatcagaa 601 tgactaaggt caagagacgt gacagcacat gctgatgcag atgtggagaa gtggaaccta 661 ctccattgct aggggagtgc agcctttaca accacactgc aagtcaatct ggcagtttct 721 caggagactt gggatagttc tgca //