LOCUS BG068430 564 bp mRNA linear EST 12-MAY-2010 DEFINITION H3065D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3065D08 3', mRNA sequence. ACCESSION BG068430 VERSION BG068430.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 564) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068430.1. Other_ESTs: H3065D08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3065 row: D column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..564 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3065D08-3" /db_xref="taxon:10090" /clone="H3065D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 149 a 134 c 122 g 159 t ORIGIN 1 attagtaatt gacttgcctg ggcttgcctg gacttgcgaa gtgtttaggc ctcgtctcct 61 taactgccaa tttgaagtct gtcatggaat cagcttagcc ttctcatgtc ttaagttgtg 121 aacatgctga agtgcgtgag ttcattttct ctaatatcct catcttcagc ttcaccaaga 181 attctttctc tatcgagtaa aaacgagaca cacaaaaagt acttcacaca agctcaggtg 241 gcagtcccca ctcctccatt cacccaggaa gagctatgga gagggtgggg gacttgagcc 301 atgaggacct tcgcctccgg tggtaggggg cttctccccc actatccagc ccaatctcct 361 gttccccaag cccagatgga aaggggtttt agctgccaga cggagaaatc cctcttcatt 421 ttggtgatcc ggtaagtgat taagaggcta cattattaaa gagcaaaaag ataatttatg 481 ctaaatgatt tcacacatag taattgtatt tgggctttgt cagcactacc acttcagcca 541 attcttgaaa gaggaaaact ctga //