LOCUS BG068422 698 bp mRNA linear EST 26-JAN-2001
DEFINITION H3065C11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3065C11 3', mRNA sequence.
ACCESSION BG068422
VERSION BG068422.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 698)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3065C11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3065 row: C column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..698
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3065C11-3"
/db_xref="taxon:10090"
/clone="H3065C11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 193 a 162 c 167 g 176 t
ORIGIN
1 gagacaaagc ctctctatgt aaccttggct attctggaac tcactctgca gactaggctg
61 gtctgcgtgc gccaccaccg cccggctggc tgcattaggg tgtttattac agcaataaac
121 actgtgacca caagcaactt ggagaggaaa ggggtttttc atcttacaac tctcaggtct
181 ctatcactga gaaagccagg acagaactca gggcagaaac taaatcagag accatggaga
241 agtgctggtt actggcttgc tcccatggct tgctcagtgt gctttatgta caacccagga
301 acacctgccc aggggtggca ctgcctagag ctggctgaac tctaccatgt cactcattaa
361 tcaagaaaat gctcaagata tgcctacagg ccagttttat aaaggcattt tattaaagtt
421 tcctcttctc aaatgacttg ctcatttaac aaggaaaatt attgcaaccc tttatcccca
481 ccatgattat agtcatttga cagacaggga caggcttggg caacagtaag tagtctgctt
541 gaaggcagat gccaaggaag taaaatgggc aaggtttgta cccaggctgc caggctccaa
601 ggctgtacct cttgcttgct gtgatatgca gacgttagaa gggaaggcag tgcttttagg
661 caagcttgtg acaagagtga agaacttgat tcaccctg
//