LOCUS BG068339 617 bp mRNA linear EST 12-MAY-2010
DEFINITION H3064D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3064D08 3', mRNA sequence.
ACCESSION BG068339
VERSION BG068339.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 617)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068339.1.
Other_ESTs: H3064D08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3064 row: D column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..617
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3064D08-3"
/db_xref="taxon:10090"
/clone="H3064D08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 181 a 126 c 97 g 213 t
ORIGIN
1 aaaaattttt ttattaaata caacttcaaa aatgtacaac ttcatattta gacatccaag
61 cttaaaattt ctttaggcta cataatctgc ccttaaaata caatacacat acacagacat
121 tagaaactca gtctccaaaa tactcagtga agatgtctgg gtgagtctta cgaaattggc
181 ctagcagttt cttcttctct ttggcagaga gttttgaatc ctcatcaata gtctttagta
241 agctcaacag ttgatctttg gttttcttct ttgtactgct agaatactca ccttcatcaa
301 ttctctggca ataaatatag cctgtgtctt tattaggatc tcttcctttt tgtatggcca
361 gaagcttaac actaacaatt ttatgtagag ctgcaggaat cttgaaaaca tctttttggt
421 gatccattaa gaagagcaca agaagatcgg ttttgccttt ggatacattt ttattgttga
481 cgatagcttt tgagaatacc cttttcacta ccatccgatt gtcactttct tcctgtaatt
541 taaattccga aggatctgct gcaacagcca tgaagtaaag cagccgtcta aactcctccc
601 tgttctggga gtcctga
//