LOCUS BG068266 691 bp mRNA linear EST 12-MAY-2010
DEFINITION H3063E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3063E10 3', mRNA sequence.
ACCESSION BG068266
VERSION BG068266.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 691)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068266.1.
Other_ESTs: H3063E10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3063 row: E column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..691
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3063E10-3"
/db_xref="taxon:10090"
/clone="H3063E10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 176 a 169 c 173 g 173 t
ORIGIN
1 agctttataa ataaaaatta cacagtatta ctttacacaa gaagagaaag aaagggtaca
61 aatctgaagt tatggtatcc agccatattg gcactggtgg tttcagactg tcagttgtct
121 tcagtctcaa gagtggagaa ggcctgaccc aggggcgtct tggtggccac cagaaccaga
181 tttctgggag agagttcagg gctgaagatg ggcaggagct cagcatagaa gccttgctcc
241 tgaagataga gcatccggtc cagtagaatc aatgtctcta ccagtggggc cagcaggagg
301 gccaagctga agaaggccac cacacgattc tcttgggcct ggtgggcctg gagggcagcc
361 agatccaaag gcagctgggg gtccagaccc actcgcttta acccttgttt cacatatctg
421 tgggaccagc cccaaatcag ttagttctca aaacttttac ctcccactta aagccgattc
481 ctactttgat ttgcttagtc attttaaaag gtcctttttg cccagcttgc cccatgctga
541 gtacaagtat cttcctagct cagcctcccc aggcaggcca cagtgcctac tctgacattc
601 ctggcttgtg tgagtgttga gatagagaat ctctgtgttg tccagactga cctctgcctc
661 ccaggatctg gggttaaaga cataagtgac t
//