LOCUS BG068088 753 bp mRNA linear EST 12-MAY-2010 DEFINITION H3061F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3061F05 3', mRNA sequence. ACCESSION BG068088 VERSION BG068088.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 753) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068088.1. Other_ESTs: H3061F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3061 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..753 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3061F05-3" /db_xref="taxon:10090" /clone="H3061F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 204 a 195 c 173 g 181 t ORIGIN 1 gtgcaccagg aagcacagat caagcctgct aaacgggatt atagctaatg gcataggaag 61 gactcatgac actggtctcg tcttgctaag acagggatgc tgagttgatc taatccagga 121 tcaaacccca gccctgccat cttacacgaa ccaatgacct ctgatcctcg tgcttcatgg 181 ctaatttgct ggcagctcac tgggactcct gcatgctgtc tggcactacg gagccttttg 241 tccttccctc ttatctatgg agaacatctg ggctttctga tgaccctgag ataggcaggg 301 ttggcactag tagtacccta acttgcagat aagaaggcaa gtttagacag cctgagcaag 361 tgatgtaagc tttggagaga cagccaagat gcagaacact gtggagttca ctcttagccc 421 tctgaatatt ggtgtgggaa tgcaaagcag ggacaggata catgaaaacc tctttgattc 481 accactattg ctggcacact tggaccttca ccagggttca tggatttgca ctgttcctga 541 cactccctac tagtgtgatc aacatctcaa gagtcaggct gcaactacaa tggcaaacca 601 ccactcagat aggagggttt catatagagc aggcaagtaa aacctctatc tgcctacacc 661 cctgccctga aagagggtag cacctctaac tgcccacacc tctgctctga aagaaggtaa 721 tacctctacc tgcagacatc ctgctctgaa aga //