LOCUS BG068086 767 bp mRNA linear EST 26-JAN-2001
DEFINITION H3061F02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3061F02 3', mRNA sequence.
ACCESSION BG068086
VERSION BG068086.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 767)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3061F02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3061 row: F column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..767
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3061F02-3"
/db_xref="taxon:10090"
/clone="H3061F02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 215 a 158 c 177 g 217 t
ORIGIN
1 ctacttacag actgctggtt agaccagcac catatattga agatgtgttg tttattgcat
61 tgtatatttt tggcttcttt gtcaaagatc aacttccgta aatgtgtggt tttatttctg
121 ggccttcaat tttattccat tgattaatat gtctgtctct gtaccagtac catgcagttt
181 ttatcactat tgcttgaggt cagggatggt gattcctcca gaagttcttt tattgttaag
241 aatcattttc actattctgg gtcttttgct tttccaggtg aatttaagaa atgctctttt
301 caacttagaa tacccaagat ataagataca atttgcaaaa cacatgaaac tcaagaagaa
361 cgaagatcaa agtgtggaat tagtgcttag aattgggaac aaaacaccca tggaaggagt
421 tacagagaca aagtttggag ctgagacgaa aggacggacc atctagagac tgccacatct
481 ggggatccat cccataatta gcctccaaac actgacacca ttgcatacac cagcaagatt
541 ttgctgaaag gacccagata tggctgtctc ttgtgagact atgccggggc ctatcaaaca
601 cagaagagga tgctcacagt cagctattgg atggatcaca ggaggtccgg gacccgccga
661 acttaggaaa ttagtctgaa caggtgagag ggtctcggac caagatggcg accgctgctg
721 ctgtggctta ggcgcctccc tagccgggcg ggcacctgga tagtcga
//