LOCUS BG068043 577 bp mRNA linear EST 12-MAY-2010 DEFINITION H3061B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3061B04 3', mRNA sequence. ACCESSION BG068043 VERSION BG068043.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 577) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068043.1. Other_ESTs: H3061B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3061 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..577 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3061B04-3" /db_xref="taxon:10090" /clone="H3061B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 123 a 124 c 152 g 178 t ORIGIN 1 aacttccggc aaattattat agattctttt agatctcata tgcatctcct tctctgaaat 61 gcatctttct ttgggtttga acaagttatc actaagagga tggggaatgg tgaactgctt 121 cttgttggcg cggacaggaa gtaggctctg cttctgcccc aggctctcct tttgttgggt 181 tttcctttgc tgtaccatgc gcttaagctt ttgcagccgt tcttgtgagc gagaaatgaa 241 ctgaggtcta tggacttcca gtgcttcctg caaagtcata gaacttgcca gcagaggctc 301 gtaactatct tctgatgggt cagagctctg tgctccctct ggggttgatg gagagggggt 361 tgctggtgag ggctcagggg tgacagtggg gtcttcgttc ttctcacatt cctttagttt 421 tacaaccaag tcacagcacg tgtaatcttt ggggaagtgt gtttctatct gaacagcacc 481 atcttcctgc agagaggcac acccagtttg taaagggcaa taaaacccag gcagagggac 541 tctgggtatc atgaagtatg ggtgttgcct ctcattg //