LOCUS BG067965 651 bp mRNA linear EST 12-MAY-2010
DEFINITION H3060C02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3060C02 3', mRNA sequence.
ACCESSION BG067965
VERSION BG067965.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 651)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067965.1.
Other_ESTs: H3060C02-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3060 row: C column: 02
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..651
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3060C02-3"
/db_xref="taxon:10090"
/clone="H3060C02"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 214 a 120 c 124 g 193 t
ORIGIN
1 attttaaatt taccaccagt ggggatgtgc tcctatgata gtgatattct gaataatcac
61 aaacactcgg tagtttccct gcatgctttc tggagccaaa ccctgtttga caacatattc
121 agtttatagg atggggcctg aaattcccat tctaaacagg acagttaggg agtgttacca
181 gtaacaaaga atgaaagtta tatgacattg attattataa attatatttg ttcttatcca
241 cccctgttgt ccttaatatg ttcgaattcc ttcttgcagg aaccatgagc ttagtagaac
301 ccagacacat ccttagtgat gattagagtg agctgtagag gctttaattc tgaagacatg
361 tatatgcagg tgagatgcaa tctgattgca aacactgaca cagtaacgaa tcaaatcaaa
421 gaaagagaag atcaaggcaa tccaaagtac tatatattca tcaatgaagc tgttaaaata
481 ctggtccaga aacagaagtg ctggacctat gaatgctgtg tcatgcaaat gcatttcact
541 tttcgtcatg tgagcaacca caagcttatt agtgatttta gccgacacaa aaccaaatgt
601 cagtatataa agacatggat gcttttcaaa aagctgaact gctgatttct t
//