LOCUS BG067961 656 bp mRNA linear EST 12-MAY-2010 DEFINITION H3060B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3060B09 3', mRNA sequence. ACCESSION BG067961 VERSION BG067961.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 656) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067961.1. Other_ESTs: H3060B09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3060 row: B column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..656 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3060B09-3" /db_xref="taxon:10090" /clone="H3060B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 222 a 146 c 87 g 201 t ORIGIN 1 accctatcct tttttaattt tagtatactg gtttttttaa aaaatataaa acaacatcat 61 ttatctatac tatcccatca gaaattccaa tttcacttaa agtttttttt tccttaagcc 121 tactcacaat gaatatctat gtatgtgaaa accccaagat tacacgtgta attactttct 181 cccttggcat aacataagta aaatacataa tatgggcagt taaatacttt tttcccaaac 241 attaggggtc atgatatata taaagattaa tttcttatgc attatcccta atgtaaaaaa 301 caaaatcaca ggtatattat actgcttgtc aaaactccgg tagtcacacc tggacactac 361 acgtccctgc tgaggctttg tgtccaccat cagtatttct gcacgagccc ttacaaccat 421 ctggagttgg aaaccaattc ccgttaaggc aaccctgtat gaaactgtcg taaaatattc 481 tgttaaggga agctgtatgt gctagacctc caacacgccg ccaggaaccc ccaaagcact 541 actctgagaa ggtaaacaac attttcacac attataaaaa atttcagctg ctctgcaacc 601 ttcctttgct aaaagttaga aacattacac cactccagca agagattaat aaataa //