LOCUS BG067913 777 bp mRNA linear EST 12-MAY-2010
DEFINITION H3059F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3059F04 3', mRNA sequence.
ACCESSION BG067913
VERSION BG067913.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 777)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067913.1.
Other_ESTs: H3059F04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3059 row: F column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..777
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3059F04-3"
/db_xref="taxon:10090"
/clone="H3059F04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 164 a 224 c 159 g 230 t
ORIGIN
1 cctcttcctc ttcctcctcc tcctcctcct ctttggacag catcttactc cgtagccctg
61 gctgtcctag aactcattct gtagaccata gtctcctgga actcagagat cagcctgcct
121 cagtgctggg attataatca taattttaat gtgtcttttt aaaattacaa tttaatttta
181 ctctgtgtac atttgtatgt gagcccacgt gtgggagtca gggcaacctg aaaaaatcag
241 tcctctcctc ccactatgta ggtcccaggg tcaaactcag gtcatcaggc ttggcagcaa
301 tcgcctgacc tgctgaacca tctcactggc cccttgaacc aacatgttct ctgtggggtg
361 cctgaccccc tcttcactct cctttcctct gcacctccag tgtttatttc tggtatgcct
421 ggaatctgtt ggcgccacag aagtagaact tggagaccct caaggccaag ggtctcactc
481 tcttccacac acacagaccc tggctctctc acctcagccc ctgcccgtag acaccatgac
541 tggagtgaca gctggagaag caggaagcag gagctgggac tcagaccagt gtgtggaatc
601 catcatggaa gcattcccat ctgctcacat ccccaggcca cacttagaac cctcaggcaa
661 tagtagccct ctttttcttt cctttttttc ttaatttatg cataatttct attcatgcat
721 acatgttcga ctgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgcacac ccatgtg
//