LOCUS BG067888 835 bp mRNA linear EST 12-MAY-2010
DEFINITION H3059D01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3059D01 3', mRNA sequence.
ACCESSION BG067888
VERSION BG067888.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 835)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067888.1.
Other_ESTs: H3059D01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3059 row: D column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..835
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3059D01-3"
/db_xref="taxon:10090"
/clone="H3059D01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 234 a 205 c 187 g 209 t
ORIGIN
1 ctcttaatta ggttcatttt aataaagagt ataggagttt acaacccaaa caattatcca
61 taaaaactat cagatacata agataaatca caccgagtga acgacagaaa cccaacagcc
121 tgcaatgaga ggtattacag taactgctag gaatgacctt ctggtttccc ctcaagattg
181 tttgcatgtt aaggattcct gaggcctgct gacctcagca ctccttccag gctgcttaga
241 gctcctcact caatcaacta caaatactgc cagcagaggg cgctcatctt ccctccttga
301 aatacgcatg gtccttggcc tctgttattt ggtttctgtt ccctaaaaat gacatcaaat
361 gttccattca aaataatgtc ttgcaggttt tgtggtgcag gccaccactg tcctcccagg
421 gctacccagg caccaagaag gtgcttactc gggaaggagg ctgaagcaat ggctggtcaa
481 ttccctcacc acagacgcaa acaacttcat ttctaacttt agaggttttg gcaaagggaa
541 cagagttgct tgtgccagct gcctgcggtc ctgtcttagc cctctgcaga tgactaagcg
601 gaggggctgg agctaacacc gtgaaggaag caggagggta catgtctgca cacacaggcg
661 ctgagaaact cctcacaaga atctttggta gacttgcaga acacagcagg atcaccactt
721 ctgaaggaaa ggattttact gtctctcaaa gtgaaggtgc tggagactgg acaagggttc
781 tcacacagtc taagcatgtg gtatcagcca acactgacct atccttgact ggtgt
//