LOCUS BG067730 672 bp mRNA linear EST 12-MAY-2010 DEFINITION H3057E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3057E10 3', mRNA sequence. ACCESSION BG067730 VERSION BG067730.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 672) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067730.1. Other_ESTs: H3057E10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3057 row: E column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..672 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3057E10-3" /db_xref="taxon:10090" /clone="H3057E10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 174 c 123 g 191 t ORIGIN 1 ctttttcggg ctgtcagtag cattataatt tgaaatgaca attttacatg acttcacact 61 cacacacaag acagtgacat agcacatagt tgtttctaaa ctctcttcac agaaggagca 121 aagatcaatc tgctctaaaa ggatgtcaac tgctatccct tacaaatctt taaaaggcag 181 cttgggtttt cctgaactga atataggcac cgtcacacct taaatggcag ggggctgtca 241 tcctgggctt gtctctagta cccagttcat acataacaaa ctgcaacctg ccttgatata 301 aaacatacca aaaccccaaa ctggcagtgg gaatcttgta accaatagct acatttcagt 361 gtagatgagt caagagttac agcccacact ctagagcaaa catctgcatc aaccaatatt 421 gtcatttctt atttcctttc tcagtgtcca agagcactac gctctctgcg tccaagctct 481 gagcactttc tatgtaaaat acacctgggt tcatgattct ttgtgacacg taaggacaac 541 agctgatcgg aacaggccgc cctgcccctg gcccccaggc ggggaagtgc ctgctccttc 601 catgtgggtc atttcactgc ttcttttttt tacttgccgg actgtccaaa atttcaatct 661 tgcctttgcc ac //