LOCUS       BG067730                 672 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3057E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3057E10 3', mRNA sequence.
ACCESSION   BG067730
VERSION     BG067730.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG067730.1.
            Other_ESTs: H3057E10-5
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3057  row: E  column: 10
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3057E10-3"
                     /db_xref="taxon:10090"
                     /clone="H3057E10"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          184 a          174 c          123 g          191 t
ORIGIN      
        1 ctttttcggg ctgtcagtag cattataatt tgaaatgaca attttacatg acttcacact
       61 cacacacaag acagtgacat agcacatagt tgtttctaaa ctctcttcac agaaggagca
      121 aagatcaatc tgctctaaaa ggatgtcaac tgctatccct tacaaatctt taaaaggcag
      181 cttgggtttt cctgaactga atataggcac cgtcacacct taaatggcag ggggctgtca
      241 tcctgggctt gtctctagta cccagttcat acataacaaa ctgcaacctg ccttgatata
      301 aaacatacca aaaccccaaa ctggcagtgg gaatcttgta accaatagct acatttcagt
      361 gtagatgagt caagagttac agcccacact ctagagcaaa catctgcatc aaccaatatt
      421 gtcatttctt atttcctttc tcagtgtcca agagcactac gctctctgcg tccaagctct
      481 gagcactttc tatgtaaaat acacctgggt tcatgattct ttgtgacacg taaggacaac
      541 agctgatcgg aacaggccgc cctgcccctg gcccccaggc ggggaagtgc ctgctccttc
      601 catgtgggtc atttcactgc ttcttttttt tacttgccgg actgtccaaa atttcaatct
      661 tgcctttgcc ac
//