LOCUS BG067617 685 bp mRNA linear EST 12-MAY-2010 DEFINITION H3056D02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3056D02 3', mRNA sequence. ACCESSION BG067617 VERSION BG067617.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 685) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067617.1. Other_ESTs: H3056D02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3056 row: D column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..685 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3056D02-3" /db_xref="taxon:10090" /clone="H3056D02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 176 a 177 c 191 g 141 t ORIGIN 1 agtggcacac acctttaatt ccagcactcc gacaacagaa gtaggtgggt ctctgtaagt 61 tcgaggccag catgttccac acagttaagt tctagaccag ccagggatgc agagtgagac 121 cctgattcga aaataaacca gtaaaactaa atatattaat taaaaacaaa gaagaggagg 181 aatagtattc ttcctggagg cccagcagaa gttagtggtt tgacaagtga tgcaaaaatg 241 gcagacattg tctccaggga gtgtggcgag tagagatctg ttcttagata gataaggtgg 301 ctcctgggac tcagccctgg ccaggacttg tgtccatagg aagaagtcac tgaggcgttg 361 agggctctgt gctccctgaa gcccgagcag cagtcacgga cactggcgtc acgggcagac 421 acagcagccc aggcactgtg tgcacagtgc gctgagaagt ttgaggtgtc acagccccaa 481 gactaccggc tcttcgtgct ggtggacggg cgctgcttcc agctggccga cgaggctctg 541 ccgcatcgca tcaagggtta tctgcttcgg agcgagccca aacgagactt ccacttcgtg 601 taccggcccc aggacagcgg caaggatgct tcaagccagc ccagtccagg acctgttgat 661 tctccaccac ccttggttcc cagca //