LOCUS BG067602 633 bp mRNA linear EST 12-MAY-2010 DEFINITION H3056B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3056B11 3', mRNA sequence. ACCESSION BG067602 VERSION BG067602.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 633) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067602.1. Other_ESTs: H3056B11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3056 row: B column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..633 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3056B11-3" /db_xref="taxon:10090" /clone="H3056B11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 131 a 146 c 154 g 202 t ORIGIN 1 tttctttttt tctttccttc cttccttcct tccttccttc cttccttctt tctttctttc 61 tttctaagta cactgtagct gtcttcagac actccagaag agggcgtcag atttcgttac 121 agatggttgt gagccaccat gtggttgctg ggatttgaac tcaggacctt tggaagagca 181 gtcggcgctc ttaaccactg agccatctca ccagccccct ggtggcactt ctaatccatg 241 ccaggtacca tgccactggc tgtactttat cacttggtct caccaaattg catagaggcc 301 ctatatattt tgctttttga gacaggggaa ctcactatgt agtggaggat aaccttgaag 361 tcttgattct cctgcttctc tctcaagacg cccgtgtaca acacatgtcg ctggtgttgt 421 gagaagagac aagatggggt gtacagcaga ggatcctggg tgagagcagc tcactcgtgg 481 gttatcttaa acatgttaat gtatacaaca taggcagtgt gaaatgtggc agtgggttag 541 gaattggatc ttttcttgag gtccattgct tacgttgcag ccaacagtct ggtggtgaag 601 agtctgggct ggggttgagt taggttctgc ctc //