LOCUS BG067589 685 bp mRNA linear EST 12-MAY-2010 DEFINITION H3056A09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3056A09 3', mRNA sequence. ACCESSION BG067589 VERSION BG067589.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 685) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067589.1. Other_ESTs: H3056A09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3056 row: A column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..685 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3056A09-3" /db_xref="taxon:10090" /clone="H3056A09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 139 a 188 c 161 g 197 t ORIGIN 1 ctctaagctt gaatcttctc aagcgtgcaa acacagttac ggattgttct gggagctaaa 61 tggctcagca tcttggcact tggttgtttt tagcttttca acaatagcca ctgttcgctc 121 ccctccatct ccatccctct gtgatgaggc aggcatccga tgaaaggctg gtcacggcgt 181 gcccccatgg tgtcagtgcg gtggcatgtg atggttcaca tggggtgtgt ttgatccaag 241 aaagcccagc ctttgcttgt ttttcctttg ttctgtcatc tgctccttca gctctgccag 301 ctgccctgac agctgcttca ccagactcat cgtagactcc agcttctcct gcaggttccg 361 gatcccattt tgctcactgt caccttcgtt gctgacaagt gacatggctc gcatccgagg 421 aaaccagtcc aaattcttct ccgtgatcat ctgagccacg tagctctcag gccctgtgta 481 ttctgtcggg tccttcacct tcaccaggac gatgaagtac aggtaatgcc acatgttgtg 541 ctctgacttg atgtgctcct caaaggacac tgtcttgtta tcaaacttgt ctctctccag 601 gccaaagcaa tctggagagg aaagggttga ttcggcttcc acaccattgt ctgtcattga 661 aggaagtcag ggcaagaacc tggag //