LOCUS BG067541 683 bp mRNA linear EST 12-MAY-2010
DEFINITION H3055E08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3055E08 3', mRNA sequence.
ACCESSION BG067541
VERSION BG067541.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 683)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067541.1.
Other_ESTs: H3055E08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3055 row: E column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..683
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3055E08-3"
/db_xref="taxon:10090"
/clone="H3055E08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 187 a 169 c 131 g 196 t
ORIGIN
1 cgtgaaaata aatttattta atgaaaaatc tacacttcct tccatgacaa gggcaaatcc
61 ccaccacaat ggagctgact tgtgaaacag atggccactc caagcttagg gtgactcact
121 catcatgtgt ccagtccagg gagagctcac ctgaggggac agccaggtgc tctttcatcc
181 cgctgactgt gctaacagac actggtgttg tgcttgctga agaagcaagc tcacagtcca
241 accagtcacg aactgagtgg cttaccactc tctagcttca agtcttaatt tggtttggaa
301 gcaaatttat ttctgtgcag accataaatt aacaaatgtc ttcaaatcca agtgtcacgt
361 tttgaaacaa atctttgttt tttcaataag catgcatctt tatctgggtt taaatattgc
421 ttacacactc tgaagtggga atatttcttt ttacatctca acattatgat gtcacaaacg
481 accagactcc tggtgaattt cattgccacg ctggcctcac aagaggctta ctcttcacac
541 tccatgtcta tgaaaagtaa ctttggaatg tcaggtccga tcagcagctc atggacaccc
601 ctccttctgg ttccacaatt cagcaggaca atctttgagc cgactgctgc tacctctggg
661 gtctctctga atgacctcag cat
//