LOCUS BG067525 609 bp mRNA linear EST 12-MAY-2010 DEFINITION H3055D04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3055D04 3', mRNA sequence. ACCESSION BG067525 VERSION BG067525.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 609) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067525.1. Other_ESTs: H3055D04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3055 row: D column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..609 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3055D04-3" /db_xref="taxon:10090" /clone="H3055D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 131 a 184 c 179 g 115 t ORIGIN 1 ggcagaagat acgtttaata acatttctta tttttaaaaa aatacagtgg caagccctcc 61 atctgtccgt caccttgcct tgcccttccc ggggctatgg cagatacagg ggtggagagg 121 ctaagtgctg ttggcctgtt cctgttcaaa cagagccatg gctagctggg tacgggcccc 181 cagcccctcc agattgctga ggcgacaacg gtggtacagt tcctcgctga gccgggggct 241 acagtcccgc agagagaact tctgcaccag ccctggctct acggctcgaa agaggtggag 301 ccctgagaac cggaggaaag catccatcac ctccagcccc tccagggctt cctcctcttc 361 ctggcctgcc agttcgccag ccagccgggc gcgggctgcc aggtagtcag cattgtagaa 421 gcagccctct gctgaagcct gccggtcaaa tctgcccccg accggagccc ccctagaagg 481 gtccacacca ggggaggggg gatcagcccc agggacacct gggggagatc tctgtggtga 541 cagagcaggg ttgaactcct gaaagtggac cggaaagaag gcctgccagc ccgagattgc 601 attcatacg //