LOCUS BG067479 500 bp mRNA linear EST 12-MAY-2010 DEFINITION H3054H04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3054H04 3', mRNA sequence. ACCESSION BG067479 VERSION BG067479.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 500) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067479.1. Other_ESTs: H3054H04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3054 row: H column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..500 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3054H04-3" /db_xref="taxon:10090" /clone="H3054H04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 100 a 137 c 139 g 124 t ORIGIN 1 cttcgtttac catttattac aaagtatgag ggtggattgg tgagcagcac cctgtaccct 61 cttctggcac agtctctgca atcaggagga aacggcatct tcccaggtgt ccaccagggg 121 gcagcacctc ccgtcgctcg gacctggttc cttcagcgag acccgcccgc ggttaactga 181 agcttcctag catcctagac cccaggcacc tcctcctcca acagagatta aggggtcctg 241 aacagcagca cagcagaaag ggcttctgat gagggcatat gtgtagtggt ggccatgttc 301 ccattgacgt gggaggtcca attcagtgtc cactcatggc tgccaccacc agagtccatg 361 cacctcaacg tggatccact tgggtcctgg catcttgagg tgatttatcg tggggcttgg 421 tcggaattgg cagggagcat gtccttgagc tcggtccttg ggtacttagc ctggttcttc 481 tgtgagttca tgtggagcta //