LOCUS BG067365 800 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053F01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053F01 3', mRNA sequence. ACCESSION BG067365 VERSION BG067365.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 800) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067365.1. Other_ESTs: H3053F01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: F column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..800 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053F01-3" /db_xref="taxon:10090" /clone="H3053F01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 196 a 206 c 186 g 212 t ORIGIN 1 attaggaatg tcttcagtcc atggatcaga acagtccagg ttccaaggta tccttagtcc 61 ctcagccaca gaacaaaacc ttcttccttt caggagttaa ctttgcatgg ggttgggatt 121 tagaacctgg aaaggctcag cagcaggagg aggataaagg tggggtctgt cacctctctc 181 cctccagtgc attcctagag aggaatgtgg aaggaagtgt tcccttagcc tcactccaag 241 gaactctaaa caagtcttct ccaggcttgc tccccatcct accttctcca gcaggctgtt 301 ttctggcttg tatcaccctt agagtttcat aattcatcat gttctggtct cccaggaaag 361 caggtcacgg gactagaaca ggatcactaa agctttccag ttcatttctt tagtgccccc 421 gaaggtgaag gtctatgcat tcattcacat catggatgct ccatcctccc agcagggggt 481 cagtgcagtc agtcaccact ggaagaagat ggaagggttt cctcagcctc tgaccttgtc 541 tcatccccac ttagcagcca gctgtggaaa cttagtccct ctgttttctg cattgttata 601 gagggacagt gaggtcctct ctgcccatgc atggttggaa gatggctcag agtctgtgca 661 gttcagtctt caaactcttc caggcacaga agtgctgatg taggatggca gagtgagctg 721 ttcagtcaag attggcagtc cattagcaat gatctctcta catccacgac aggcacccca 781 agttctcaaa aaagtcaaca //