LOCUS BG067330 677 bp mRNA linear EST 12-MAY-2010 DEFINITION H3053B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3053B11 3', mRNA sequence. ACCESSION BG067330 VERSION BG067330.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 677) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067330.1. Other_ESTs: H3053B11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3053 row: B column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..677 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3053B11-3" /db_xref="taxon:10090" /clone="H3053B11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 164 c 200 g 147 t ORIGIN 1 actggaaact aattgcaaaa tttatttcag ggctgcaggg ctgcaggggt ttataagggc 61 acttcttgtc ttggggctgt gagtctctgg ccttggtggg agcacgcatt tcaggggcag 121 aggtgggggc tgagccaggg tagtagaaga caggagacag ccttgtccag ttgggtggac 181 ctagcataag ctgtaaatct gacgcgaaag caggaaagac agtggatgtc cagtctggaa 241 cagaggatgg cttaggcctc tcccccgagt gcttggtcct acagagtggc tttcaggagg 301 caaagcagca gccagggaac tgaagccagc ttcaagggta gtggcgaggg accaaaagga 361 tggagccatg agaaagtcct cagcactgtc ctcatataaa gatctgtgag ttctcctaga 421 tttacccata tccccacctg cacggactct aagccccagc aggcctcctg ttgttcccct 481 agtggggctg aggcagaggg caagcaatag cacttctcgg gggtgaggtg gggtacagat 541 gccaccccga ctcttggaaa gagatgtcag gagaaagctt tgatgctagt ccccatatcc 601 cagctcagtc cccagtctgt ctcctgccca cttctctctc acacactttc caaggactat 661 ctaggtgaaa ggaaaag //