LOCUS BG067222 527 bp mRNA linear EST 12-MAY-2010 DEFINITION H3051H11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3051H11 3', mRNA sequence. ACCESSION BG067222 VERSION BG067222.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 527) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067222.1. Other_ESTs: H3051H11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3051 row: H column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..527 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3051H11-3" /db_xref="taxon:10090" /clone="H3051H11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 120 c 148 g 112 t ORIGIN 1 cattgttttt attaattggc tttataggtt aaagtgcata gtaaagacaa aaaaaggaaa 61 tgcatacaat aagaaagggg ctttcctaga caacattaga aaggacctga gatgcctaat 121 ttttttctaa ggaacatgga aagagatgca gatgcaggca gtaggcctgg agtcggggcc 181 tgcagaagct gctggcatgc taaccctacc caggcaaagg ctgcaaggga gggtgcctgg 241 ataaagactc acaggaggtg ctgctgacac tgaacctgct gacaccacca ccaccacaga 301 acctctcctg cagagagaca catccacccc tgctatctga gtgcaagttt gacgagggtg 361 caggggtcac tcagcttgtg tctgctgtga aagccccgcc ccactgggga cagtgagtat 421 ataactgggc catggagagt tgaagtgcct gtcacaggcc tccatctcca ggagggcagg 481 agcccaagga gtagggtgcc tgggttcttg tgctccttga aggtaaa //