LOCUS BG067156 462 bp mRNA linear EST 12-MAY-2010 DEFINITION H3051C01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3051C01 3', mRNA sequence. ACCESSION BG067156 VERSION BG067156.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 462) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067156.1. Other_ESTs: H3051C01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3051 row: C column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..462 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3051C01-3" /db_xref="taxon:10090" /clone="H3051C01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 125 a 119 c 114 g 104 t ORIGIN 1 atttttattg gagaatatct gatgcttttg gaatgcactt aagatatacc tgtagtccaa 61 ctccacatgt gcaacacagg aagcagcttc gtgaggtgga cagtgagcgc agccagatct 121 gcctgcaccg ttccacaccg acactcctgt gtgcaactgt cctgagcacg agacgggcct 181 ggcaagctca agtgttcctc tcaagctatt ttccaggcca tgccaagagt aaactccttg 241 tatcaggtca gtgtccctgc aaggagctgc tgacaggtag atcaggtaaa gcagcgtgga 301 gacagacact gagtatattc ttcctgaacg ggacaaaagt cagaaagaga caaaaggact 361 tcagtgcccc gtgagtcctc tggggttcaa tgacaacaca gcacaggctg agaacagggt 421 tgcaatcatt cctgatccct cacaacctct cgtcctggga ca //