LOCUS BG067126 734 bp mRNA linear EST 12-MAY-2010 DEFINITION H3050H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3050H05 3', mRNA sequence. ACCESSION BG067126 VERSION BG067126.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 734) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067126.1. Other_ESTs: H3050H05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3050 row: H column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..734 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3050H05-3" /db_xref="taxon:10090" /clone="H3050H05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 228 a 178 c 144 g 184 t ORIGIN 1 gtttatttat ttattacaat ttatcacaaa tacaaatcaa gggatgtagc tcagtgatag 61 aagagtgcat gcttcccatt cacaaaattc tgggcttgat gcccagaact ccaaaatgac 121 acacagagca catgaatgaa tgaatgaatg aatgaataca caaaccagca ccaaataccc 181 aaaaagaaac acagatatac atgtgtgcac cagccagcaa gcttagtgga aactcaagtg 241 tccaagagag ctggctaggg tcactgtgga ggtataactg gtcgagatca tgggccatgt 301 ggctgaactt aatctctatc cttgtcccat tctggcattc aggctaagcc ccaaccctca 361 atacatacgg ctggtctttc tggagggttg gcctccatcc tgagtcaaag ttcaacaaat 421 tctggaatgc tctgagaccc ccaacatgaa taattacaac acttctatca ctggggaatc 481 ctcaaaggtt tagtctccct ctctggagct gagatagaaa tatactaatt tttagaatgc 541 tggagatgac ttgtatccca ggagatagag agaccatgta tcttcattaa gatggcccta 601 agacctgctg ctctcactgt ggctcacttc ccagccctta ccacagattc atccaaccaa 661 ccattaaaga gacagatgag acaccagctc ctcattgttc ccaacagttg ccctagtacc 721 tgatgaactt cagg //