LOCUS BG067113 794 bp mRNA linear EST 12-MAY-2010 DEFINITION H3050G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3050G04 3', mRNA sequence. ACCESSION BG067113 VERSION BG067113.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 794) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067113.1. Other_ESTs: H3050G04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3050 row: G column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..794 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3050G04-3" /db_xref="taxon:10090" /clone="H3050G04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 199 c 214 g 199 t ORIGIN 1 cacattaaca tgtgtttatt cagtttcttc catctaccat aaatctggac agtccatcag 61 atacaaggca agttgtcaca ccactgtaga tcacataaaa caagggatgt ctggggtttg 121 ctgcatctta tctggaaggg tgctgcttct taggcccagg ccaccgtggc attgctggtt 181 gctttagtct ggctgctgct acccactcac gaatgagggt agattccagc ttacgcacct 241 ccacgacaga tggggggtct tctgagttag atgctctgag atctaggtga tgtgctcccc 301 cctggatggt gacagcaatg actgatgtac tcaggttgct ctggatcccg ccccctgccc 361 aggggtctag gtcaccgttg gagaaaatga tgttgctggc tgctttaagg tcaccacccc 421 agaagctggt ctgcagccag tcttgccgag gccacacgcc ccatgtatcc agacagtatt 481 gctggcggag ttcttcagag aatgggattt caggaaacct gtctgtcaca ttgttgctgt 541 caaaggtcag attgatctcc gtacaggcct gccagtgccc gtagacccat gatcctctgg 601 ccctcattca acagccgttg acagcccacc ttgacagggt tggcaggaag gggccctaga 661 aagtcagtag gatacgggta gtccatcatg gcgagcacag taaatgcatt tcgggcaaac 721 caaagagctg agtcaggtcc tttgggctgg aaagtgattg acaggtacaa agttctggct 781 gatggtgtca tagg //