LOCUS BG067081 732 bp mRNA linear EST 12-MAY-2010 DEFINITION H3050D04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3050D04 3', mRNA sequence. ACCESSION BG067081 VERSION BG067081.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 732) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067081.1. Other_ESTs: H3050D04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3050 row: D column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..732 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3050D04-3" /db_xref="taxon:10090" /clone="H3050D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 160 a 213 c 156 g 203 t ORIGIN 1 atgttataaa atagccttta atttttccct ctcacaagat atcactgatt aaactggagc 61 ggcactcagt tccctagggc tggtccttgt gaggaaggaa ggtagcagac ccggctgctg 121 tgcaggccca agtaccaggc cccaagctta gtacacactc aggaacaaac acccacgatg 181 cgctgcggtt caggggctct ggagttctgg gccagcagtg tagtctctgt acgggctgag 241 tgcaccaggc actcctgtct gtgagctgtt tcctctccct gtcttactgc tgcccctcat 301 cctgcagttc tcaactctga gaagtactaa ggagcgccct gccctgccac ggacctccga 361 ctcctatcac tgcaggcttg aatcctgttt tgctttacat ttgcttcaga cttcctggag 421 gcctgaccca catgaagatg acctgctcca cccaggacgc ttagaagcca cttccatttt 481 ccttcccaca tgacagttcc ttttaacgtc tctgtcacag tggacaggtc tgaattcaag 541 agtgaggact ggaccctcgt tctccaagtc gtctgttttg ttcatgtttt ctttaacatg 601 ctcatctgca tgtgcacagc tctggaaaga tcctcttgtc tcaagaatat caaccacttc 661 ccaatcatgg attcattcct ttcaggaact gatattcaaa ccacagactg gtctctgcct 721 tcccagatgt ct //