LOCUS BG067057 609 bp mRNA linear EST 12-MAY-2010 DEFINITION H3050A12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3050A12 3', mRNA sequence. ACCESSION BG067057 VERSION BG067057.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 609) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067057.1. Other_ESTs: H3050A12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3050 row: A column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..609 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3050A12-3" /db_xref="taxon:10090" /clone="H3050A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 206 a 119 c 125 g 159 t ORIGIN 1 aatgtgcatt gcatcacagt tccactcatg tatttgcata ttatttatag ctgtaacagc 61 agaaatgagt aataagagag ctacatgact ggcagacctt aaaacactct ctggctgttt 121 acagaaaagc ttgctggcta tttatagagc aaacagaata cttgtattgg ggtatggagc 181 taaagaacag aggcatgctt tccagtgtcc ctgaactacg agtgacttga aacagctcct 241 ggtcagatgc tgatgactta aatgctctgc agacaggctg tgcggactac agcaactcta 301 tcatctacct atcagtgagc agaccatact ccagatctgt gtataactgt acatatacac 361 agctaagcaa aaacagacga ggcaggagcc agccaatccc aggataaaga gcccagagag 421 aagaaggaac ctaaagttac atctattatg ctctagtaac tgggttagag aaattttagt 481 atgttttctc tatatgcgag aaatacagat gccaccgtta ttaggaagta tactcgagaa 541 aaaacaattt acaactgcac agtgtaccca tgaattaagg gacaattttt cttttagata 601 taaagtaac //