LOCUS BG067008 834 bp mRNA linear EST 12-MAY-2010 DEFINITION H3049E08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3049E08 3', mRNA sequence. ACCESSION BG067008 VERSION BG067008.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 834) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067008.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3049 row: E column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..834 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3049E08-3" /db_xref="taxon:10090" /clone="H3049E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 286 a 208 c 142 g 198 t ORIGIN 1 ggtttttcta gacagggtag cccccttgct gtcctggaac atactctgta tgtagaccag 61 actcagaaat ccacctgcct ctgcctccca agcgctggga ttaaagccgt gcaccgggcc 121 attagtccat tcctaggtga gtaactataa caaaagaacc tcaaggttcc caaagccaaa 181 acctaccaca tcacccaaag acagctgtac tgttttaaca gctacattaa atgcacagaa 241 cttgaagaaa cacacccttc atatctttcg acaaagttcc tcataatatc tggacaagga 301 caaagatgct cgatgttcaa gcttaatgtt acttcctttc aaactatcaa tcacaaatgt 361 aaacccttac aagcaaattt caccctaccc accacaccat attcaaaagg tggttaaacc 421 ccaagtttca actaagggta aatctgttta gtgcaaagaa catttgacaa tgtttacagg 481 agaggtggct actgtcacct acagcaaaac tgtttgttag tggtctagta tggaaaggac 541 aggctctcaa caacaacaaa aaaaaaccca ccatcagtgc tcaggtttgc tagaaagcct 601 gtcacttaca aaatctcaaa taaacactta gatcatagta aatcaaaccc agaaaagtgg 661 ctactttctg cagccaaaaa ctgaatacat cataacctgc tcaagtattc acaaatggga 721 cagtaagaca tcatgtgcaa gttgctgatc cagaactaag gcgtgggcag ctttgagaag 781 ccacacttcc tgagtcatat gactacagtg taaacctcac tgcacacaca aaaa //