LOCUS BG066903 583 bp mRNA linear EST 12-MAY-2010 DEFINITION H3048D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3048D03 3', mRNA sequence. ACCESSION BG066903 VERSION BG066903.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 583) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066903.1. Other_ESTs: H3048D03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3048 row: D column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..583 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3048D03-3" /db_xref="taxon:10090" /clone="H3048D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 193 a 94 c 98 g 198 t ORIGIN 1 atagcacatg acttcattta atattgatag cagctcaatt taacagttga aaaagcaaac 61 acctttaata aatttggtgt gaatgatcat atagcctgat atactattgt acaggagtgt 121 atacatataa atctatatgt tgaattatgt acatttcaat atataccaca gatttttcct 181 actcacaatt ttcttttgat aaaggaagca gtttcactgt acacgatgtg aaatgctaca 241 ttatgtcctg agtaattgag ggattattaa taaactactg aaaattctct ctctcttttt 301 agttaatgat cttgaaggtt ctgtactaaa acatggacaa aggatattta aaagattgca 361 tccagctttg cacttgtttt atgcagaaaa agctaaaaaa tgaacaacag cctttgaggt 421 ttgggaaaat gccctcaaat agtgaaggtt aaacaatgtc ttatttgtag ggatttctgc 481 agtttctgca atgttgccta catccaattt gtcaggcatg gaagactctt ctcatgattg 541 gcctacagat cctttttact gaacaggaca ttctatgtaa acg //