LOCUS BG066798 757 bp mRNA linear EST 12-MAY-2010 DEFINITION H3047A10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3047A10 3', mRNA sequence. ACCESSION BG066798 VERSION BG066798.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 757) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066798.1. Other_ESTs: H3047A10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3047 row: A column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..757 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3047A10-3" /db_xref="taxon:10090" /clone="H3047A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 216 a 184 c 153 g 204 t ORIGIN 1 ctctggtttt tttcgagaca gggtttctct gtgtagccct ggctgtcctg gaactcactt 61 tgtagaccag gccggccttg aactcagaaa tctgcctgcc tctgcctccc aagtgctggg 121 attaaaggag tgcgccacca cgcccggctt ttgtgtgata tttctaattt gtatgttctt 181 tacaatgaag tcctaataat aaattataaa aacttagcaa acaaatgcca cttgccatgt 241 aggaaactgg ggcatacttc cggttaagcg actccacctc ctctaggacg cgactgtaca 301 cggacatcat tcttctctca tactcactgt cggcgtgggc agcgcccgtg gacccatact 361 cctggaagct gagcagagac cagcagttca tccatcctag caccttcatc acctccatgc 421 atttgctaac catggctcac attctcacct catcttttta aaaaatgaag ttatttccaa 481 ttacagataa ggaaactgag gcttgttaag attaatctga ctacacttgt gtaactatta 541 agtcacaagt tgtaactcaa acccaaatct gactacaaat gacataattc ttagctattc 601 acatgaaggc tatttgtcac catacaggaa agacacttga gaaagggcca gcaggtaaca 661 tactgatatg acatcatata agtaacagat ggaacacagg gagcaggtta gcaaatcact 721 tggtcagcat gccattagac ctggggatgg ctgaaga //