LOCUS BG066798 757 bp mRNA linear EST 12-MAY-2010
DEFINITION H3047A10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3047A10 3', mRNA sequence.
ACCESSION BG066798
VERSION BG066798.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 757)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066798.1.
Other_ESTs: H3047A10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3047 row: A column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..757
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3047A10-3"
/db_xref="taxon:10090"
/clone="H3047A10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 216 a 184 c 153 g 204 t
ORIGIN
1 ctctggtttt tttcgagaca gggtttctct gtgtagccct ggctgtcctg gaactcactt
61 tgtagaccag gccggccttg aactcagaaa tctgcctgcc tctgcctccc aagtgctggg
121 attaaaggag tgcgccacca cgcccggctt ttgtgtgata tttctaattt gtatgttctt
181 tacaatgaag tcctaataat aaattataaa aacttagcaa acaaatgcca cttgccatgt
241 aggaaactgg ggcatacttc cggttaagcg actccacctc ctctaggacg cgactgtaca
301 cggacatcat tcttctctca tactcactgt cggcgtgggc agcgcccgtg gacccatact
361 cctggaagct gagcagagac cagcagttca tccatcctag caccttcatc acctccatgc
421 atttgctaac catggctcac attctcacct catcttttta aaaaatgaag ttatttccaa
481 ttacagataa ggaaactgag gcttgttaag attaatctga ctacacttgt gtaactatta
541 agtcacaagt tgtaactcaa acccaaatct gactacaaat gacataattc ttagctattc
601 acatgaaggc tatttgtcac catacaggaa agacacttga gaaagggcca gcaggtaaca
661 tactgatatg acatcatata agtaacagat ggaacacagg gagcaggtta gcaaatcact
721 tggtcagcat gccattagac ctggggatgg ctgaaga
//