LOCUS BG066786 768 bp mRNA linear EST 12-MAY-2010
DEFINITION H3046H09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3046H09 3', mRNA sequence.
ACCESSION BG066786
VERSION BG066786.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 768)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG066786.1.
Other_ESTs: H3046H09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3046 row: H column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..768
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3046H09-3"
/db_xref="taxon:10090"
/clone="H3046H09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 298 a 123 c 117 g 230 t
ORIGIN
1 cttttaaaaa tacacacatt taatgttggt ctgttttaat tctaagtaaa gcaatttcaa
61 aaatacaaac tgaacatcag agccatgtaa accaccatga taaaagaaaa cagacattca
121 caataattaa gtctagagac agttcagctt gataaattgt cttccaagta tcttaaagaa
181 ttactaaaaa tactgctggt gttcagttaa aattaaagaa ctttgggaaa aatgaaatat
241 gatacagctg gagtgaataa ggttacacgg gaattacatt ttccattacc cctagatata
301 aacaaacatt caaaaattaa cacaatctct gtgttattca aaatattgca gctgcgggaa
361 aggcaacttt aaattaatag ctaggggaaa tcaagttttg gtggaggtga acagaagtga
421 cagtttgtaa tttgcacttt gtaaagctca ttatacctgg ttaaccagga accaaaagca
481 ggacaagaaa ttctctgctt cttttggcta caatgtgaca agaaaagtca tcttttgaag
541 atgcttaagg aaataaagca tgtttcttta aaaacaggca aataatcaat taatggaata
601 aataagcact aaccacaatt ttagcattct gtcatcaata cacgtcacat attttttcat
661 tttggcagaa atttccccaa tttttagtct aaaatccacc aatcattata aaagtaaaat
721 gaagagctac caaaataata ttcttctgtt tactctttgg ctaagaaa
//