LOCUS BG066661 659 bp mRNA linear EST 12-MAY-2010 DEFINITION H3044F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3044F05 3', mRNA sequence. ACCESSION BG066661 VERSION BG066661.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 659) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG066661.1. Other_ESTs: H3044F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3044 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..659 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3044F05-3" /db_xref="taxon:10090" /clone="H3044F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 197 a 129 c 159 g 174 t ORIGIN 1 aagataaaga atccatttta ttctaatttg gaccttgtga gaaaaaaatg actgattaga 61 tggagcaatc cagtaatcaa aggaagaata gtttgcagag ctattaccga ggtggtgttg 121 accttgcatt ttgaagtttc tcatcaagag atgggtcctt ctgtgttgcc aaggctggcc 181 atgagatccc aggctcaagc agtctttctg ccccagcctc ctaagcagct gggactacag 241 gtatgtgctg caacctggga ggagctatga cattaagcaa gggctccctg aacatcacca 301 tcttttctgc aagtgccttg gaggccttca tctgacggca agcttgggaa gtttggttgc 361 aaagttccat gtgcgttagt caaactactt atttaataga aagacaattt tgtatccact 421 attatacagg aaattaaaaa ataataattg tgagaaagaa aattcctaaa gaaaatgttt 481 aacctttact gaagtgaaag gcaggtttct ggtggggtgg agtcctcaaa tggcgctgct 541 atgggccccg acgcagtgcc tgttattgaa acaggtggag aatgctatga agaaaagagg 601 gaaaaatagc cccctacccc cctctaaaga cagggtttca ctcactggct ggactggaa //