LOCUS BG066624 748 bp mRNA linear EST 26-JAN-2001
DEFINITION H3044C04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3044C04 3', mRNA sequence.
ACCESSION BG066624
VERSION BG066624.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 748)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3044C04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3044 row: C column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..748
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3044C04-3"
/db_xref="taxon:10090"
/clone="H3044C04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 210 a 151 c 168 g 219 t
ORIGIN
1 gagttaagtg attggatgaa tctcagaaaa gattttaagc ttcagacttt aaacattgtg
61 gacattttga tagactatgg gacctttgaa ggtggactaa atgtattttt gcgttatgct
121 atacctaggc atggccccta cagactcatg tgtttaaaca agcacatggg acccagggag
181 tggactgtgg tactttgaat attcttggcc aatgggaagt ggcactgtta ggaaagatgg
241 ccttgataga gtccacatgt agccttatta gaagaagtac atcactgtgg gtggctttga
301 agctccaccc agtgcaaaag aaccagtctt cttctatttg ccgccttctt taaacaaaaa
361 aaaaaaaaaa aagatttttt ttttatttta ttttatgtat gagtacaaca ttgctctctc
421 cagacacacc agaagagggt gtcagatccc attacagatg gttgtgagcc accatgtggt
481 tgctgggaac tgaactcagg acctctggag gggcagtctg tgctcttaac tgttgagcca
541 cctgtccagc cctcccattt gtagatgatg tagatgtaga tgtagaactc tcagatcctc
601 tagtgccatg tctgcctgca cactgccatg cttctgccac gatggagtga acttctaaac
661 ctgtaagcca gctccaatta aatattgtcc tttataagag tatcattggt catggggtcc
721 ttacagcaat ggaaccctaa ataggaca
//